Optimization of culture conditions for antibacterial substance production from newly isolated Brevibacillus laterosporus EA62

Objectives: In the present study, it was reported effects of some nutritional (amino acid, carbon, nitrogen and metal sources) physical factors (pH temperature) on antibacterial substance activity Brevibacillus laterosporus EA62. Methods: The agar well diffusion assay performed to evaluate substance. new examined against four pathogenic bacteria under different conditions. Results: best obtained in modified medium consists 5% glucose, 0.1% tryptone, 0.05% MgSO4+CaCO3 0.5% glutamic acid. For parameters, maximal observed after 72 h when incubated at 37°C, pH 7.0. Conclusions: This study indicates that EA62 could be an important source substances this optimization.